biostudies-literatureYamada YMedical Research CouncilWellcome Trust ProgramWellcome Trust390-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC2845816biostudies-literatureUnknown339(2)The mature fruiting body of Dictyostelium consists of stalk and spore cells but its construction, and the migration of the preceding slug stage, requires a number of specialized sub-types of prestalk cell whose nature and function are not well understood. The prototypic prestalk-specific gene, ecmA, is inducible by the polyketide DIF-1 in a monolayer assay and requires the DimB and MybE transcription factors for full inducibility. We perform genome-wide microarray analyses, on parental, mybE- and dimB- cells, and identify many additional genes that depend on MybE and DimB for their DIF-1 inducibility. Surprisingly, an even larger number of genes are only DIF inducible in mybE- cells, some genes are only inducible in DimB- cells and some are inducible when either transcription factor is absDevelopmental biologyA new Dictyostelium prestalk cell sub-type.PMC2845816053640/ZMC_U105115237053640Williams JGKay RRBloomfield GYamada YIvens ARoss SfalseA new Dictyostelium prestalk cell sub-type.The mature fruiting body of Dictyostelium consists of stalk and spore cells but its construction, and the migration of the preceding slug stage, requires a number of specialized sub-types of prestalk cell whose nature and function are not well understood. The prototypic prestalk-specific gene, ecmA, is inducible by the polyketide DIF-1 in a monolayer assay and requires the DimB and MybE transcription factors for full inducibility. We perform genome-wide microarray analyses, on parental, mybE- and dimB- cells, and identify many additional genes that depend on MybE and DimB for their DIF-1 inducibility. Surprisingly, an even larger number of genes are only DIF inducible in mybE- cells, some genes are only inducible in DimB- cells and some are inducible when either transcription factor is abs2010-01-01T00:00:00Z2010 Mar2026-04-18T11:11:19.735Z2026-04-07T14:35:33.368ZS-EPMC28458162008008510.1016/j.ydbio.2009.12.045