{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Joyce CM"],"funding":["NIGMS NIH HHS"],"pagination":["1032-40"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2846202"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["1804(5)"],"pubmed_abstract":["A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway."],"journal":["Biochimica et biophysica acta"],"pubmed_title":["Techniques used to study the DNA polymerase reaction pathway."],"pmcid":["PMC2846202"],"funding_grant_id":["R01 GM028550","GM-28550","R01 GM028550-29"],"pubmed_authors":["Joyce CM"],"additional_accession":[]},"is_claimable":false,"name":"Techniques used to study the DNA polymerase reaction pathway.","description":"A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 May","modification":"2025-06-01T01:08:17.955Z","creation":"2019-03-27T00:29:43Z"},"accession":"S-EPMC2846202","cross_references":{"pubmed":["19665596"],"doi":["10.1016/j.bbapap.2009.07.021"]}}