biostudies-literatureJoyce CMNIGMS NIH HHS1032-40https://www.ebi.ac.uk/biostudies/studies/S-EPMC2846202biostudies-literatureUnknown1804(5)A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.Biochimica et biophysica actaTechniques used to study the DNA polymerase reaction pathway.PMC2846202R01 GM028550GM-28550R01 GM028550-29Joyce CMfalseTechniques used to study the DNA polymerase reaction pathway.A minimal reaction pathway for DNA polymerases was established over 20years ago using chemical-quench methods. Since that time there has been considerable interest in noncovalent steps in the reaction pathway, conformational changes involving the polymerase or its DNA substrate that may play a role in substrate specificity. Fluorescence-based assays have been devised in order to study these conformational transitions and the results obtained have added new detail to the reaction pathway.2010-01-01T00:00:00Z2010 May2025-06-01T01:08:17.955Z2019-03-27T00:29:43ZS-EPMC28462021966559610.1016/j.bbapap.2009.07.021