{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wiederkehr A"],"funding":["NCI NIH HHS","NIGMS NIH HHS"],"pagination":["4770-82"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC284782"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(12)"],"pubmed_abstract":["Sec3p is a component of the exocyst complex that tethers secretory vesicles to the plasma membrane at exocytic sites in preparation for fusion. Unlike all other exocyst structural genes, SEC3 is not essential for growth. Cells lacking Sec3p grow and secrete surprisingly well at 25 degrees C; however, late markers of secretion, such as the vesicle marker Sec4p and the exocyst subunit Sec8p, localize more diffusely within the bud. Furthermore, sec3Delta cells are strikingly round relative to wild-type cells and are unable to form pointed mating projections in response to alpha factor. These phenotypes support the proposed role of Sec3p as a spatial landmark for secretion. We also find that cells lacking Sec3p exhibit a dramatic defect in the inheritance of cortical ER into the bud, whereas t"],"journal":["Molecular biology of the cell"],"pubmed_title":["Sec3p is needed for the spatial regulation of secretion and for the inheritance of the cortical endoplasmic reticulum."],"pmcid":["PMC284782"],"funding_grant_id":["GM35370","R01 GM035370","R37 GM035370","P01 CA046128","CA 46128"],"pubmed_authors":["Wiederkehr A","Ferro-Novick S","Du Y","Novick P","Pypaert M"],"additional_accession":[]},"is_claimable":false,"name":"Sec3p is needed for the spatial regulation of secretion and for the inheritance of the cortical endoplasmic reticulum.","description":"Sec3p is a component of the exocyst complex that tethers secretory vesicles to the plasma membrane at exocytic sites in preparation for fusion. Unlike all other exocyst structural genes, SEC3 is not essential for growth. Cells lacking Sec3p grow and secrete surprisingly well at 25 degrees C; however, late markers of secretion, such as the vesicle marker Sec4p and the exocyst subunit Sec8p, localize more diffusely within the bud. Furthermore, sec3Delta cells are strikingly round relative to wild-type cells and are unable to form pointed mating projections in response to alpha factor. These phenotypes support the proposed role of Sec3p as a spatial landmark for secretion. We also find that cells lacking Sec3p exhibit a dramatic defect in the inheritance of cortical ER into the bud, whereas t","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Dec","modification":"2025-04-04T11:13:28.643Z","creation":"2019-03-27T00:36:03Z"},"accession":"S-EPMC284782","cross_references":{"pubmed":["12960429"],"doi":["10.1091/mbc.e03-04-0229"]}}