biostudies-literatureFolli FNIDDK NIH HHSe9923https://www.ebi.ac.uk/biostudies/studies/S-EPMC2848014biostudies-literatureUnknown5(3)<h4>Background</h4>In type 1 diabetes (T1D) vascular complications such as accelerated atherosclerosis and diffused macro-/microangiopathy are linked to chronic hyperglycemia with a mechanism that is not yet well understood. End-stage renal disease (ESRD) worsens most diabetic complications, particularly, the risk of morbidity and mortality from cardiovascular disease is increased several fold.<h4>Methods and findings</h4>We evaluated protein regulation and expression in skin biopsies obtained from T1D patients with and without ESRD, to identify pathways of persistent cellular changes linked to diabetic vascular disease. We therefore examined pathways that may be normalized by restoration of normoglycemia with kidney-pancreas (KP) transplantation. Using proteomic and ultrastructural approaPloS oneProteomics reveals novel oxidative and glycolytic mechanisms in type 1 diabetic patients' skin which are normalized by kidney-pancreas transplantation.PMC2848014R01 DK079195Guzzi VLa Rosa SSocci CCapella CBachi AFolli FLauro DBrownlee MPerego LFinzi GParoni RTripathy DFiorina PCighetti GOrsenigo EColetta DKGregorini LPlacidi CStaudacher CJenkinson CSecchi AfalseProteomics reveals novel oxidative and glycolytic mechanisms in type 1 diabetic patients' skin which are normalized by kidney-pancreas transplantation.<h4>Background</h4>In type 1 diabetes (T1D) vascular complications such as accelerated atherosclerosis and diffused macro-/microangiopathy are linked to chronic hyperglycemia with a mechanism that is not yet well understood. End-stage renal disease (ESRD) worsens most diabetic complications, particularly, the risk of morbidity and mortality from cardiovascular disease is increased several fold.<h4>Methods and findings</h4>We evaluated protein regulation and expression in skin biopsies obtained from T1D patients with and without ESRD, to identify pathways of persistent cellular changes linked to diabetic vascular disease. We therefore examined pathways that may be normalized by restoration of normoglycemia with kidney-pancreas (KP) transplantation. Using proteomic and ultrastructural approa2010-01-01T00:00:00Z2010 Mar2026-05-05T05:00:24.07Z2019-03-26T23:07:18ZS-EPMC28480142036086710.1371/journal.pone.0009923