biostudies-literatureKang MNIDDK NIH HHS1226-35https://www.ebi.ac.uk/biostudies/studies/S-EPMC2848927biostudies-literatureUnknown159(6)<h4>Background and purpose</h4>Excitation-transcriptional coupling involves communication between plasma membrane ion channels and gene expression in the nucleus. Calcium influx through L-type Ca(2+) channels induces phosphorylation of the transcription factor, cyclic-AMP response element binding protein (CREB) and downstream activation of the cyclic-AMP response element (CRE) promoter regions. Tyrosine nitration of Ca(2+) channels attenuates interactions with c-Src kinase, decreasing Ca(2+) channel currents and smooth muscle contraction during colonic inflammation. In this study we examined the effect of tyrosine nitration and colonic inflammation on Ca(2+) channel mediated phosphorylation of CREB and CRE activation.<h4>Experimental approach</h4>CREB and phospho-CREB were detected by WestBritish journal of pharmacologyThe effect of tyrosine nitration of L-type Ca2+ channels on excitation-transcription coupling in colonic inflammation.PMC2848927R01 DK046367DK046367Kang MAkbarali HIRoss GRfalseThe effect of tyrosine nitration of L-type Ca2+ channels on excitation-transcription coupling in colonic inflammation.<h4>Background and purpose</h4>Excitation-transcriptional coupling involves communication between plasma membrane ion channels and gene expression in the nucleus. Calcium influx through L-type Ca(2+) channels induces phosphorylation of the transcription factor, cyclic-AMP response element binding protein (CREB) and downstream activation of the cyclic-AMP response element (CRE) promoter regions. Tyrosine nitration of Ca(2+) channels attenuates interactions with c-Src kinase, decreasing Ca(2+) channel currents and smooth muscle contraction during colonic inflammation. In this study we examined the effect of tyrosine nitration and colonic inflammation on Ca(2+) channel mediated phosphorylation of CREB and CRE activation.<h4>Experimental approach</h4>CREB and phospho-CREB were detected by West2010-01-01T00:00:00Z2010 Mar2025-06-01T00:53:52.622Z2019-06-06T21:58:35ZS-EPMC28489272012881010.1111/j.1476-5381.2009.00599.x