{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["106(2)"],"submitter":["Klein HP"],"pubmed_abstract":["In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected."],"journal":["Journal of bacteriology"],"pagination":["596-602"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC285135"],"repository":["biostudies-literature"],"pubmed_title":["Variations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells."],"pmcid":["PMC285135"],"pubmed_authors":["Klein HP","Jahnke L"],"additional_accession":[]},"is_claimable":false,"name":"Variations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells.","description":"In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.","dates":{"release":"1971-01-01T00:00:00Z","publication":"1971 May","modification":"2025-04-04T09:50:14.487Z","creation":"2025-02-19T04:02:30.261Z"},"accession":"S-EPMC285135","cross_references":{"pubmed":["4102333"],"doi":["10.1128/jb.106.2.596-602.1971"]}}