biostudies-literatureUnknown106(2)Klein HPIn cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.Journal of bacteriology596-602https://www.ebi.ac.uk/biostudies/studies/S-EPMC285135biostudies-literatureVariations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells.PMC285135Klein HPJahnke LfalseVariations in the localization of acetyl-coenzyme A synthetase in aerobic yeast cells.In cells of Saccharomyces cerevisiae growing aerobically for 24 hr, acetyl-coenzyme A synthetase [acetate: CoA ligase (AMP), EC 6.2.1.1] was localized principally in the microsomal fraction. On density gradients, the enzyme in such cells behaved as a low-density particle, readily separable from the soluble proteins. After 48 hr of incubation, the cells showed a bimodal distribution of enzyme, with most of the activity now sedimenting with the mitochondrial fraction and only a smaller amount with the microsomal fraction. By using density gradients, two forms of synthetase were obtained from these cells: one band denser and the other band less dense than the intact mitochondria. In all preparations containing synthetase activity, appreciable levels of phospholipids were also detected.1971-01-01T00:00:00Z1971 May2025-04-04T09:50:14.487Z2025-02-19T04:02:30.261ZS-EPMC285135410233310.1128/jb.106.2.596-602.1971