{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["66(Pt 4)"],"submitter":["Hehemann JH"],"pubmed_abstract":["Marine bacteria secrete specific glycoside hydrolases such as agarases to access polysaccharides from algal cell walls as a carbon and energy source. In an attempt to identify agarases with variable degradation patterns, a novel family GH16 beta-agarase from the marine bacterium Zobellia galactanivorans was expressed, purified and crystallized. The purified enzyme crystallized in two distinct forms that were grown by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. Hexagonal crystals belonging to space group P3(1)21 diffracted to 2.2 A resolution, whereas orthorhombic crystals belonging to space group P2(1)2(1)2(1) diffracted to 1.5 A resolution."],"journal":["Acta crystallographica. Section F, Structural biology and crystallization communications"],"pagination":["413-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2852333"],"repository":["biostudies-literature"],"pubmed_title":["Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans."],"pmcid":["PMC2852333"],"pubmed_authors":["Barbeyron T","Czjzek M","Hehemann JH","Michel G"],"additional_accession":[]},"is_claimable":false,"name":"Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans.","description":"Marine bacteria secrete specific glycoside hydrolases such as agarases to access polysaccharides from algal cell walls as a carbon and energy source. In an attempt to identify agarases with variable degradation patterns, a novel family GH16 beta-agarase from the marine bacterium Zobellia galactanivorans was expressed, purified and crystallized. The purified enzyme crystallized in two distinct forms that were grown by the hanging-drop vapour-diffusion method using polyethylene glycol as a precipitant. Hexagonal crystals belonging to space group P3(1)21 diffracted to 2.2 A resolution, whereas orthorhombic crystals belonging to space group P2(1)2(1)2(1) diffracted to 1.5 A resolution.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Apr","modification":"2026-05-01T15:52:17.041Z","creation":"2026-04-07T17:04:22.667Z"},"accession":"S-EPMC2852333","cross_references":{"pubmed":["20383011"],"doi":["10.1107/s174430911000429x","10.1107/S174430911000429X"]}}