{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Das R"],"funding":["NIGMS NIH HHS"],"pagination":["291-4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2854559"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(4)"],"pubmed_abstract":["We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6-20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain."],"journal":["Nature methods"],"pubmed_title":["Atomic accuracy in predicting and designing noncanonical RNA structure."],"pmcid":["PMC2854559"],"funding_grant_id":["P20 GM076222-03S1","P20 GM076222"],"pubmed_authors":["Das R","Baker D","Karanicolas J"],"additional_accession":[]},"is_claimable":false,"name":"Atomic accuracy in predicting and designing noncanonical RNA structure.","description":"We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6-20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Apr","modification":"2024-11-12T11:37:57.872Z","creation":"2019-03-27T00:30:07Z"},"accession":"S-EPMC2854559","cross_references":{"pubmed":["20190761"],"doi":["10.1038/nmeth.1433"]}}