biostudies-literatureDas RNIGMS NIH HHS291-4https://www.ebi.ac.uk/biostudies/studies/S-EPMC2854559biostudies-literatureUnknown7(4)We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6-20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain.Nature methodsAtomic accuracy in predicting and designing noncanonical RNA structure.PMC2854559P20 GM076222-03S1P20 GM076222Das RBaker DKaranicolas JfalseAtomic accuracy in predicting and designing noncanonical RNA structure.We present fragment assembly of RNA with full-atom refinement (FARFAR), a Rosetta framework for predicting and designing noncanonical motifs that define RNA tertiary structure. In a test set of thirty-two 6-20-nucleotide motifs, FARFAR recapitulated 50% of the experimental structures at near-atomic accuracy. Sequence redesign calculations recovered native bases at 65% of residues engaged in noncanonical interactions, and we experimentally validated mutations predicted to stabilize a signal recognition particle domain.2010-01-01T00:00:00Z2010 Apr2024-11-12T11:37:57.872Z2019-03-27T00:30:07ZS-EPMC28545592019076110.1038/nmeth.1433