{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kosugi T"],"funding":["NCRR NIH HHS"],"pagination":["498-503"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2856804"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["166(3)"],"pubmed_abstract":["The highly conserved DRY motif located at the end of the third transmembrane of G-protein-coupled receptors has been described as a key motif for several aspects of GPCR functions. However, in the case of the vertebrate gonadotropin-releasing hormone receptor (GnRHR), the amino acid in the third position in the DRY motif is variable. In the lamprey, a most basal vertebrate, the third amino acid of the \"DRY\" in lamprey (lGnRHR-1) is His, while it is most often His/Gln in the type II GnRHR. To investigate the functional significance of the substitution of DRY to DRH in the GnRHR-1, second messenger signaling, ligand binding and internalization of the wild-type and mutant lGnRH receptors were characterized with site-directed mutagenesis. Treatment of the DRE(151) and DRS(151) mutant receptors"],"journal":["General and comparative endocrinology"],"pubmed_title":["A role of Histidine151 in the lamprey gonadotropin-releasing hormone receptor-1 (lGnRHR-1): Functional insight of diverse amino acid residues in the position of Tyr of the DRY motif in GnRHR from an ancestral type II receptor."],"pmcid":["PMC2856804"],"funding_grant_id":["R21 RR024477-02","5R21RR024477-02","R21 RR024477"],"pubmed_authors":["Kosugi T","Sower SA"],"additional_accession":[]},"is_claimable":false,"name":"A role of Histidine151 in the lamprey gonadotropin-releasing hormone receptor-1 (lGnRHR-1): Functional insight of diverse amino acid residues in the position of Tyr of the DRY motif in GnRHR from an ancestral type II receptor.","description":"The highly conserved DRY motif located at the end of the third transmembrane of G-protein-coupled receptors has been described as a key motif for several aspects of GPCR functions. However, in the case of the vertebrate gonadotropin-releasing hormone receptor (GnRHR), the amino acid in the third position in the DRY motif is variable. In the lamprey, a most basal vertebrate, the third amino acid of the \"DRY\" in lamprey (lGnRHR-1) is His, while it is most often His/Gln in the type II GnRHR. To investigate the functional significance of the substitution of DRY to DRH in the GnRHR-1, second messenger signaling, ligand binding and internalization of the wild-type and mutant lGnRH receptors were characterized with site-directed mutagenesis. Treatment of the DRE(151) and DRS(151) mutant receptors","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 May","modification":"2025-04-19T23:55:20.611Z","creation":"2019-06-06T22:00:12Z"},"accession":"S-EPMC2856804","cross_references":{"pubmed":["20005226"],"doi":["10.1016/j.ygcen.2009.12.001"]}}