{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kao H"],"funding":["NIAID NIH HHS"],"pagination":["8248-57"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2857686"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["181(12)"],"pubmed_abstract":["The mechanism underlying the transient accumulation of CD4 at the immunological synapse (IS) and its significance for T cell activation are not understood. To investigate these issues, we mutated a serine phosphorylation site (S408) in the cytoplasmic tail of murine CD4. Preventing phosphorylation of S408 did not block CD4 recruitment to the IS; rather, it blocked the ability of CD4 to leave the IS. Surprisingly, enhanced and prolonged CD4 accumulation at the supramolecular activation cluster in the contact area had no functional consequence for T cell activation, cytokine production, or proliferation. Protein kinase C theta (PKCtheta)-deficient T cells also displayed enhanced and prolonged accumulation of wild-type CD4 at the IS, indicating that theta is the critical PKC isoform involved "],"journal":["Journal of immunology (Baltimore, Md. : 1950)"],"pubmed_title":["Regulated movement of CD4 in and out of the immunological synapse."],"pmcid":["PMC2857686"],"funding_grant_id":["P01 AI071195","AI071195","R37 AI024157","R37 AI024157-21","P01 AI071195-03","AI24157","R01 AI024157"],"pubmed_authors":["Littman DR","Allen PM","Lin J","Shaw AS","Kao H"],"additional_accession":[]},"is_claimable":false,"name":"Regulated movement of CD4 in and out of the immunological synapse.","description":"The mechanism underlying the transient accumulation of CD4 at the immunological synapse (IS) and its significance for T cell activation are not understood. To investigate these issues, we mutated a serine phosphorylation site (S408) in the cytoplasmic tail of murine CD4. Preventing phosphorylation of S408 did not block CD4 recruitment to the IS; rather, it blocked the ability of CD4 to leave the IS. Surprisingly, enhanced and prolonged CD4 accumulation at the supramolecular activation cluster in the contact area had no functional consequence for T cell activation, cytokine production, or proliferation. Protein kinase C theta (PKCtheta)-deficient T cells also displayed enhanced and prolonged accumulation of wild-type CD4 at the IS, indicating that theta is the critical PKC isoform involved ","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Dec","modification":"2025-04-04T13:38:35.201Z","creation":"2019-03-26T23:42:52Z"},"accession":"S-EPMC2857686","cross_references":{"pubmed":["19050241"],"doi":["10.4049/jimmunol.181.12.8248"]}}