biostudies-literatureRichardson MRNCRR NIH HHSNHLBI NIH HHSNIAAA NIH HHS2468-83https://www.ebi.ac.uk/biostudies/studies/S-EPMC2859442biostudies-literatureUnknown9(9)Although low-density lipoprotein (LDL) plays a predominant role in atherogenesis, the low-density lipoproteome has not been fully characterized. Moreover, alterations from a Western diet, diabetes, and physical inactivity on this proteome have yet to be determined. Accordingly, relative quantification was determined in LDL proteins from male Yucatan diabetic dyslipidemic (DD) swine in the early stages of atherosclerosis compared to healthy control (C) and non-diabetic hyperlipidemic (H) swine. Importantly, coronary vascular dysfunction was prevented by aerobic exercise training in these animals (DDX) without altering total LDL concentration. Using 2-DE, Western blot, label-free quantitative MS, and selected reaction monitoring, alterations in the abundance of apolipoproteins A-I, B, C-III,ProteomicsDiabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs.PMC2859442R24 RR013223RR013223R21 AA015698R01 HL047586AA015698R01 HL062552R01 HL062552-08HL47586HL062552Lai XSturek MRichardson MRDixon JLWitzmann FAfalseDiabetic dyslipidemia and exercise alter the plasma low-density lipoproteome in Yucatan pigs.Although low-density lipoprotein (LDL) plays a predominant role in atherogenesis, the low-density lipoproteome has not been fully characterized. Moreover, alterations from a Western diet, diabetes, and physical inactivity on this proteome have yet to be determined. Accordingly, relative quantification was determined in LDL proteins from male Yucatan diabetic dyslipidemic (DD) swine in the early stages of atherosclerosis compared to healthy control (C) and non-diabetic hyperlipidemic (H) swine. Importantly, coronary vascular dysfunction was prevented by aerobic exercise training in these animals (DDX) without altering total LDL concentration. Using 2-DE, Western blot, label-free quantitative MS, and selected reaction monitoring, alterations in the abundance of apolipoproteins A-I, B, C-III,2009-01-01T00:00:00Z2009 May2025-06-01T12:00:34.358Z2019-03-27T00:30:20ZS-EPMC28594421940204610.1002/pmic.200800613