{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["98(9)"],"submitter":["Japrung D"],"pubmed_abstract":["The staphylococcal alpha-hemolysin (alphaHL) protein nanopore is under investigation as a fast, cheap detector for nucleic acid analysis and sequencing. Although discrimination of all four bases of DNA by the alphaHL pore has been demonstrated, analysis of single-stranded DNAs and RNAs containing secondary structure mediated by basepairing is prevented because these nucleic acids cannot be translocated through the pore. Here, we show that a structured 95-nucleotide single-stranded DNA and its RNA equivalent are translocated through the alphaHL pore in the presence of 4 M urea, a concentration that denatures the secondary structure of the polynucleotides. The alphaHL pore is functional even in 7 M urea, and therefore it is easily stable enough for analyses of challenging DNA and RNA species."],"journal":["Biophysical journal"],"pagination":["1856-63"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2862201"],"repository":["biostudies-literature"],"pubmed_title":["Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore."],"pmcid":["PMC2862201"],"pubmed_authors":["Henricus M","Maglia G","Bayley H","Japrung D","Li Q"],"additional_accession":[]},"is_claimable":false,"name":"Urea facilitates the translocation of single-stranded DNA and RNA through the alpha-hemolysin nanopore.","description":"The staphylococcal alpha-hemolysin (alphaHL) protein nanopore is under investigation as a fast, cheap detector for nucleic acid analysis and sequencing. Although discrimination of all four bases of DNA by the alphaHL pore has been demonstrated, analysis of single-stranded DNAs and RNAs containing secondary structure mediated by basepairing is prevented because these nucleic acids cannot be translocated through the pore. Here, we show that a structured 95-nucleotide single-stranded DNA and its RNA equivalent are translocated through the alphaHL pore in the presence of 4 M urea, a concentration that denatures the secondary structure of the polynucleotides. The alphaHL pore is functional even in 7 M urea, and therefore it is easily stable enough for analyses of challenging DNA and RNA species.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 May","modification":"2025-04-03T23:23:46.569Z","creation":"2019-03-27T00:30:27Z"},"accession":"S-EPMC2862201","cross_references":{"pubmed":["20441749"],"doi":["10.1016/j.bpj.2009.12.4333"]}}