{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Freel SA"],"funding":["Intramural NIH HHS","NIAID NIH HHS","PHS HHS"],"pagination":["4998-5006"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2863846"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84(10)"],"pubmed_abstract":["Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding the ability of vaccine strategies to elicit them. We used multiparameter flow cytometry and viable cell sorts from phenotypically defined CD8(+) T-cell subsets in combination with a highly standardized virus inhibition assay to evaluate CD8(+) T-cell-mediated inhibition of viral replication. Here we show that vaccination against HIV-1 Env and Gag-Pol by DNA priming followed by recombinant adenovirus type 5 (rAd5) boosting elicited CD8(+) T-cell-mediated antiviral activity aga"],"journal":["Journal of virology"],"pubmed_title":["Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination."],"pmcid":["PMC2863846"],"funding_grant_id":["U01 AI067854","P30 AI027767","Z99 AI999999","AI067854-05","R01A5779","U19 AI067854","P30 AI064518","P30 AI27767","P30 AI 64518"],"pubmed_authors":["Zarkowsky D","Cunningham CK","Chattopadhyay PK","Roederer M","Ferrari G","Lamoreaux L","Saunders K","Ochsenbauer C","Graham BS","Kappes JC","Koup RA","Edmonds TG","Overman RG","Haynes BF","Weinhold KJ","Freel SA","Denny TN","Tomaras GD"],"additional_accession":[]},"is_claimable":false,"name":"Phenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.","description":"Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding the ability of vaccine strategies to elicit them. We used multiparameter flow cytometry and viable cell sorts from phenotypically defined CD8(+) T-cell subsets in combination with a highly standardized virus inhibition assay to evaluate CD8(+) T-cell-mediated inhibition of viral replication. Here we show that vaccination against HIV-1 Env and Gag-Pol by DNA priming followed by recombinant adenovirus type 5 (rAd5) boosting elicited CD8(+) T-cell-mediated antiviral activity aga","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 May","modification":"2025-04-04T11:56:02.057Z","creation":"2019-03-27T00:30:33Z"},"accession":"S-EPMC2863846","cross_references":{"pubmed":["20200250"],"doi":["10.1128/JVI.00138-10","10.1128/jvi.00138-10"]}}