biostudies-literatureFreel SAIntramural NIH HHSNIAID NIH HHSPHS HHS4998-5006https://www.ebi.ac.uk/biostudies/studies/S-EPMC2863846biostudies-literatureUnknown84(10)Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding the ability of vaccine strategies to elicit them. We used multiparameter flow cytometry and viable cell sorts from phenotypically defined CD8(+) T-cell subsets in combination with a highly standardized virus inhibition assay to evaluate CD8(+) T-cell-mediated inhibition of viral replication. Here we show that vaccination against HIV-1 Env and Gag-Pol by DNA priming followed by recombinant adenovirus type 5 (rAd5) boosting elicited CD8(+) T-cell-mediated antiviral activity agaJournal of virologyPhenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.PMC2863846U01 AI067854P30 AI027767Z99 AI999999AI067854-05R01A5779U19 AI067854P30 AI064518P30 AI27767P30 AI 64518Zarkowsky DCunningham CKChattopadhyay PKRoederer MFerrari GLamoreaux LSaunders KOchsenbauer CGraham BSKappes JCKoup RAEdmonds TGOverman RGHaynes BFWeinhold KJFreel SADenny TNTomaras GDfalsePhenotypic and functional profile of HIV-inhibitory CD8 T cells elicited by natural infection and heterologous prime/boost vaccination.Control of HIV-1 replication following nonsterilizing HIV-1 vaccination could be achieved by vaccine-elicited CD8(+) T-cell-mediated antiviral activity. To date, neither the functional nor the phenotypic profiles of CD8(+) T cells capable of this activity are clearly understood; consequently, little is known regarding the ability of vaccine strategies to elicit them. We used multiparameter flow cytometry and viable cell sorts from phenotypically defined CD8(+) T-cell subsets in combination with a highly standardized virus inhibition assay to evaluate CD8(+) T-cell-mediated inhibition of viral replication. Here we show that vaccination against HIV-1 Env and Gag-Pol by DNA priming followed by recombinant adenovirus type 5 (rAd5) boosting elicited CD8(+) T-cell-mediated antiviral activity aga2010-01-01T00:00:00Z2010 May2025-04-04T11:56:02.057Z2019-03-27T00:30:33ZS-EPMC28638462020025010.1128/JVI.00138-1010.1128/jvi.00138-10