biostudies-literature00480Shin SNIAID NIH HHSNINDS NIH HHS156-62https://www.ebi.ac.uk/biostudies/studies/S-EPMC2864485biostudies-literatureUnknown303(2)Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP synthase beta-subunit and actin proteins from the surface biotinylated HBMEC as the mannose-insensitive binding targets for FimH. Co-immunoprecipitation experiments in the presence of alpha-methyl mannose verified the binding of FimH to ATP synthase beta-subunit of HBMEC. ATP synthase beta-subunit antibody decreased E. coli K1 binding to HBMEC in the presence of alpha-methyl mannose. Taken together, these findings demonstrate that FimH of E. coli K1 binds to HBMEC in both mannose-sensitive and -insensitive manner.FEMS microbiology lettersHuman brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH.PMC2864485R01 AI047225R01 AI047225-05NS 26310R01 NS026310R01 NS026310-21AI 47225R56 NS026310Shin SKim KS48falseHuman brain endothelial ATP synthase beta-subunit is mannose-insensitive binding target of FimH.Binding of meningitis-causing Escherichia coli K1 to human brain microvascular endothelial cells (HBMEC) contributes to traversal of the blood-brain barrier, which occurs in part by the mannose-sensitive binding of FimH. In this study, we showed that FimH also binds to HBMEC, independent of mannose, and identified ATP synthase beta-subunit and actin proteins from the surface biotinylated HBMEC as the mannose-insensitive binding targets for FimH. Co-immunoprecipitation experiments in the presence of alpha-methyl mannose verified the binding of FimH to ATP synthase beta-subunit of HBMEC. ATP synthase beta-subunit antibody decreased E. coli K1 binding to HBMEC in the presence of alpha-methyl mannose. Taken together, these findings demonstrate that FimH of E. coli K1 binds to HBMEC in both mannose-sensitive and -insensitive manner.2010-01-01T00:00:00Z2010 Feb2024-12-04T03:09:03.963Z2019-03-27T00:30:34ZS-EPMC28644852006753010.1111/j.1574-6968.2009.01878.x