{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Joergensen LM"],"pubmed_abstract":["<h4>Background</h4>Infected humans make protective antibody responses to the PfEMP1 adhesion antigens exported by Plasmodium falciparum parasites to the erythrocyte membrane, but little is known about the kinetics of this antibody-receptor binding reaction or how the topology of PfEMP1 on the parasitized erythrocyte membrane influences antibody association with, and dissociation from, its antigenic target.<h4>Methods</h4>A Quartz Crystal Microbalance biosensor was used to measure the association and dissociation kinetics of VAR2CSA PfEMP1 binding to human monoclonal antibodies. Immuno-fluorescence microscopy was used to visualize antibody-mediated adhesion between the surfaces of live infected erythrocytes and atomic force microscopy was used to obtain higher resolution images of the membr"],"journal":["Malaria journal"],"pagination":["100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2868858"],"repository":["biostudies-literature"],"pubmed_title":["The kinetics of antibody binding to Plasmodium falciparum VAR2CSA PfEMP1 antigen and modelling of PfEMP1 antigen packing on the membrane knobs."],"pmcid":["PMC2868858"],"pubmed_authors":["Joergensen LM","Dobrilovic T","Arnot DE","Barfod L","Hviid L","Theander TG","Salanti A","Hassenkam T"],"additional_accession":[]},"is_claimable":false,"name":"The kinetics of antibody binding to Plasmodium falciparum VAR2CSA PfEMP1 antigen and modelling of PfEMP1 antigen packing on the membrane knobs.","description":"<h4>Background</h4>Infected humans make protective antibody responses to the PfEMP1 adhesion antigens exported by Plasmodium falciparum parasites to the erythrocyte membrane, but little is known about the kinetics of this antibody-receptor binding reaction or how the topology of PfEMP1 on the parasitized erythrocyte membrane influences antibody association with, and dissociation from, its antigenic target.<h4>Methods</h4>A Quartz Crystal Microbalance biosensor was used to measure the association and dissociation kinetics of VAR2CSA PfEMP1 binding to human monoclonal antibodies. Immuno-fluorescence microscopy was used to visualize antibody-mediated adhesion between the surfaces of live infected erythrocytes and atomic force microscopy was used to obtain higher resolution images of the membr","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Apr","modification":"2026-05-07T16:06:09.404Z","creation":"2026-04-07T23:01:21.472Z"},"accession":"S-EPMC2868858","cross_references":{"pubmed":["20403153"],"doi":["10.1186/1475-2875-9-100"]}}