{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["59(6)"],"submitter":["Grouwels G"],"pubmed_abstract":["<h4>Objective</h4>Generating functional beta-cells by inducing their proliferation may provide new perspectives for cell therapy in diabetes. Transcription factor E2F1 controls G(1)- to S-phase transition during the cycling of many cell types and is required for pancreatic beta-cell growth and function. However, the consequences of overexpression of E2F1 in beta-cells are unknown.<h4>Research design and methods</h4>The effects of E2F1 overexpression on beta-cell proliferation and function were analyzed in isolated rat beta-cells and in transgenic mice.<h4>Results</h4>Adenovirus AdE2F1-mediated overexpression of E2F1 increased the proliferation of isolated primary rat beta-cells 20-fold but also enhanced beta-cell death. Coinfection with adenovirus AdAkt expressing a constitutively active f"],"journal":["Diabetes"],"pagination":["1435-44"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2874704"],"repository":["biostudies-literature"],"pubmed_title":["Ectopic expression of E2F1 stimulates beta-cell proliferation and function."],"pmcid":["PMC2874704"],"pubmed_authors":["Leuckx G","Hoebeke I","Chintinne M","Heimberg H","Cai Y","Ziebold U","Van de Casteele M","Grouwels G","Stange G","Ling Z","Pipeleers D","Heremans Y"],"additional_accession":[]},"is_claimable":false,"name":"Ectopic expression of E2F1 stimulates beta-cell proliferation and function.","description":"<h4>Objective</h4>Generating functional beta-cells by inducing their proliferation may provide new perspectives for cell therapy in diabetes. Transcription factor E2F1 controls G(1)- to S-phase transition during the cycling of many cell types and is required for pancreatic beta-cell growth and function. However, the consequences of overexpression of E2F1 in beta-cells are unknown.<h4>Research design and methods</h4>The effects of E2F1 overexpression on beta-cell proliferation and function were analyzed in isolated rat beta-cells and in transgenic mice.<h4>Results</h4>Adenovirus AdE2F1-mediated overexpression of E2F1 increased the proliferation of isolated primary rat beta-cells 20-fold but also enhanced beta-cell death. Coinfection with adenovirus AdAkt expressing a constitutively active f","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jun","modification":"2025-04-26T06:20:02.583Z","creation":"2019-03-27T00:30:58Z"},"accession":"S-EPMC2874704","cross_references":{"pubmed":["20299467"],"doi":["10.2337/db09-1295"]}}