biostudies-literatureUnknown59(6)Grouwels G<h4>Objective</h4>Generating functional beta-cells by inducing their proliferation may provide new perspectives for cell therapy in diabetes. Transcription factor E2F1 controls G(1)- to S-phase transition during the cycling of many cell types and is required for pancreatic beta-cell growth and function. However, the consequences of overexpression of E2F1 in beta-cells are unknown.<h4>Research design and methods</h4>The effects of E2F1 overexpression on beta-cell proliferation and function were analyzed in isolated rat beta-cells and in transgenic mice.<h4>Results</h4>Adenovirus AdE2F1-mediated overexpression of E2F1 increased the proliferation of isolated primary rat beta-cells 20-fold but also enhanced beta-cell death. Coinfection with adenovirus AdAkt expressing a constitutively active fDiabetes1435-44https://www.ebi.ac.uk/biostudies/studies/S-EPMC2874704biostudies-literatureEctopic expression of E2F1 stimulates beta-cell proliferation and function.PMC2874704Leuckx GHoebeke IChintinne MHeimberg HCai YZiebold UVan de Casteele MGrouwels GStange GLing ZPipeleers DHeremans YfalseEctopic expression of E2F1 stimulates beta-cell proliferation and function.<h4>Objective</h4>Generating functional beta-cells by inducing their proliferation may provide new perspectives for cell therapy in diabetes. Transcription factor E2F1 controls G(1)- to S-phase transition during the cycling of many cell types and is required for pancreatic beta-cell growth and function. However, the consequences of overexpression of E2F1 in beta-cells are unknown.<h4>Research design and methods</h4>The effects of E2F1 overexpression on beta-cell proliferation and function were analyzed in isolated rat beta-cells and in transgenic mice.<h4>Results</h4>Adenovirus AdE2F1-mediated overexpression of E2F1 increased the proliferation of isolated primary rat beta-cells 20-fold but also enhanced beta-cell death. Coinfection with adenovirus AdAkt expressing a constitutively active f2010-01-01T00:00:00Z2010 Jun2025-04-26T06:20:02.583Z2019-03-27T00:30:58ZS-EPMC28747042029946710.2337/db09-1295