biostudies-literatureWilliams LMNIEHS NIH HHSNHGRI NIH HHSPHS HHS32https://www.ebi.ac.uk/biostudies/studies/S-EPMC2874759biostudies-literatureUnknown11<h4>Background</h4>By targeting SNPs contained in both coding and non-coding areas of the genome, we are able to identify genetic differences and characterize genome-wide patterns of variation among individuals, populations and species. We investigated the utility of 454 sequencing and MassARRAY genotyping for population genetics in natural populations of the teleost, Fundulus heteroclitus as well as closely related Fundulus species (F. grandis, F. majalis and F. similis).<h4>Results</h4>We used 454 pyrosequencing and MassARRAY genotyping technology to identify and type 458 genome-wide SNPs and determine genetic differentiation within and between populations and species of Fundulus. Specifically, pyrosequencing identified 96 putative SNPs across coding and non-coding regions of the F. heteBMC geneticsSNP identification, verification, and utility for population genetics in a non-model genus.PMC2874759DEB0948510ES525163R01 HG003229R01 ES011588R01 HG003229-05Boyko ARMa XOleksiak MFWilliams LMBustamante CDfalseSNP identification, verification, and utility for population genetics in a non-model genus.<h4>Background</h4>By targeting SNPs contained in both coding and non-coding areas of the genome, we are able to identify genetic differences and characterize genome-wide patterns of variation among individuals, populations and species. We investigated the utility of 454 sequencing and MassARRAY genotyping for population genetics in natural populations of the teleost, Fundulus heteroclitus as well as closely related Fundulus species (F. grandis, F. majalis and F. similis).<h4>Results</h4>We used 454 pyrosequencing and MassARRAY genotyping technology to identify and type 458 genome-wide SNPs and determine genetic differentiation within and between populations and species of Fundulus. Specifically, pyrosequencing identified 96 putative SNPs across coding and non-coding regions of the F. hete2010-01-01T00:00:00Z2010 Apr2025-04-26T06:22:01.289Z2019-03-27T00:30:58ZS-EPMC28747592043372610.1186/1471-2156-11-32