{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Park DJ"],"funding":["American Heart Association","NIDDK NIH HHS","National Institutes of Health"],"pagination":["2831-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2877844"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["176(6)"],"pubmed_abstract":["Heme oxygenase-1 (HO-1) is a microsomal enzyme with antioxidant, antiapoptotic, and immunoregulatory functions. We studied the expression of HO-1 by bone marrow-derived dendritic cells (BMDCs) and splenic DC subpopulations under quiescent conditions or following lipopolysaccharide (LPS) stimulation. The kinetics of HO-1 expression by BMDCs depended on the conditions under which they were propagated. Expression of HO-1 in mouse BMDCs in 100 U/ml GM-CSF peaked at 16 hours after LPS treatment and maintained expression for at least 48 hours. But cultures in 800 U/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) showed peak expression by 16 hours that disappeared by 48 hours after LPS stimulation, similar to BMDCs cultured in both 100 U/ml GM-CSF and IL-4 (10 ng/ml). By flow cytomet"],"journal":["The American journal of pathology"],"pubmed_title":["Heme oxygenase-1 expression in murine dendritic cell subpopulations: effect on CD8+ dendritic cell differentiation in vivo."],"pmcid":["PMC2877844"],"funding_grant_id":["R01 DK059600","0655318B","DK59600","P30 DK 079337","DK 079337","P30 DK079337","DK75332"],"pubmed_authors":["Agarwal A","George JF","Park DJ"],"additional_accession":[]},"is_claimable":false,"name":"Heme oxygenase-1 expression in murine dendritic cell subpopulations: effect on CD8+ dendritic cell differentiation in vivo.","description":"Heme oxygenase-1 (HO-1) is a microsomal enzyme with antioxidant, antiapoptotic, and immunoregulatory functions. We studied the expression of HO-1 by bone marrow-derived dendritic cells (BMDCs) and splenic DC subpopulations under quiescent conditions or following lipopolysaccharide (LPS) stimulation. The kinetics of HO-1 expression by BMDCs depended on the conditions under which they were propagated. Expression of HO-1 in mouse BMDCs in 100 U/ml GM-CSF peaked at 16 hours after LPS treatment and maintained expression for at least 48 hours. But cultures in 800 U/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) showed peak expression by 16 hours that disappeared by 48 hours after LPS stimulation, similar to BMDCs cultured in both 100 U/ml GM-CSF and IL-4 (10 ng/ml). By flow cytomet","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jun","modification":"2025-04-04T11:31:38.488Z","creation":"2019-03-27T00:31:08Z"},"accession":"S-EPMC2877844","cross_references":{"pubmed":["20395442"],"doi":["10.2353/ajpath.2010.090845"]}}