{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sharma R"],"funding":["NIDCR NIH HHS"],"pagination":["e10895"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2878349"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(5)"],"pubmed_abstract":["<h4>Background</h4>It is not known why the ameloblasts responsible for dental enamel formation are uniquely sensitive to fluoride (F(-)). Herein, we present a novel theory with supporting data to show that the low pH environment of maturating stage ameloblasts enhances their sensitivity to a given dose of F(-). Enamel formation is initiated in a neutral pH environment (secretory stage); however, the pH can fall to below 6.0 as most of the mineral precipitates (maturation stage). Low pH can facilitate entry of F(-) into cells. Here, we asked if F(-) was more toxic at low pH, as measured by increased cell stress and decreased cell function.<h4>Methodology/principal findings</h4>Treatment of ameloblast-derived LS8 cells with F(-) at low pH reduced the threshold dose of F(-) required to phosph"],"journal":["PloS one"],"pubmed_title":["The acid test of fluoride: how pH modulates toxicity."],"pmcid":["PMC2878349"],"funding_grant_id":["DE018106","R01 DE018106"],"pubmed_authors":["Skobe Z","Tsuchiya M","Sharma R","Tannous BA","Bartlett JD"],"additional_accession":[]},"is_claimable":false,"name":"The acid test of fluoride: how pH modulates toxicity.","description":"<h4>Background</h4>It is not known why the ameloblasts responsible for dental enamel formation are uniquely sensitive to fluoride (F(-)). Herein, we present a novel theory with supporting data to show that the low pH environment of maturating stage ameloblasts enhances their sensitivity to a given dose of F(-). Enamel formation is initiated in a neutral pH environment (secretory stage); however, the pH can fall to below 6.0 as most of the mineral precipitates (maturation stage). Low pH can facilitate entry of F(-) into cells. Here, we asked if F(-) was more toxic at low pH, as measured by increased cell stress and decreased cell function.<h4>Methodology/principal findings</h4>Treatment of ameloblast-derived LS8 cells with F(-) at low pH reduced the threshold dose of F(-) required to phosph","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 May","modification":"2026-05-09T03:15:43.748Z","creation":"2019-03-26T23:07:33Z"},"accession":"S-EPMC2878349","cross_references":{"pubmed":["20531944"],"doi":["10.1371/journal.pone.0010895"]}}