{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["38(10)"],"submitter":["Lee KM"],"pubmed_abstract":["TRAP150 has been identified as a subunit of the transcription regulatory complex TRAP/Mediator, and also a component of the spliceosome. The exact function of TRAP150, however, remains unclear. We recently identified TRAP150 by its association with the mRNA export factor TAP. TRAP150 contains an arginine/serine-rich domain and has sequence similarity with the cell death-promoting transcriptional repressor BCLAF1. We found that TRAP150 co-localizes with splicing factors in nuclear speckles, and is required for pre-mRNA splicing and activates splicing in vivo. TRAP150 remains associated with the spliced mRNA after splicing, and accordingly, it interacts with the integral exon junction complex. Unexpectedly, when tethered to a precursor mRNA, TRAP150 can trigger mRNA degradation in the nucleus. However, unlike nonsense-mediated decay, TRAP150-mediated mRNA decay is irrespective of the presence of upstream stop codons and occurs in the nucleus. Moreover, TRAP150 activates pre-mRNA splicing and induces mRNA degradation by its separable functional domains. Therefore, TRAP150 represents a multi-functional protein involved in nuclear mRNA metabolism."],"journal":["Nucleic acids research"],"pagination":["3340-50"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2879504"],"repository":["biostudies-literature"],"pubmed_title":["TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation."],"pmcid":["PMC2879504"],"pubmed_authors":["Tarn WY","Hsu IaW","Lee KM"],"additional_accession":[]},"is_claimable":false,"name":"TRAP150 activates pre-mRNA splicing and promotes nuclear mRNA degradation.","description":"TRAP150 has been identified as a subunit of the transcription regulatory complex TRAP/Mediator, and also a component of the spliceosome. The exact function of TRAP150, however, remains unclear. We recently identified TRAP150 by its association with the mRNA export factor TAP. TRAP150 contains an arginine/serine-rich domain and has sequence similarity with the cell death-promoting transcriptional repressor BCLAF1. We found that TRAP150 co-localizes with splicing factors in nuclear speckles, and is required for pre-mRNA splicing and activates splicing in vivo. TRAP150 remains associated with the spliced mRNA after splicing, and accordingly, it interacts with the integral exon junction complex. Unexpectedly, when tethered to a precursor mRNA, TRAP150 can trigger mRNA degradation in the nucleus. However, unlike nonsense-mediated decay, TRAP150-mediated mRNA decay is irrespective of the presence of upstream stop codons and occurs in the nucleus. Moreover, TRAP150 activates pre-mRNA splicing and induces mRNA degradation by its separable functional domains. Therefore, TRAP150 represents a multi-functional protein involved in nuclear mRNA metabolism.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jun","modification":"2025-04-22T06:42:02.764Z","creation":"2019-03-27T00:31:14Z"},"accession":"S-EPMC2879504","cross_references":{"pubmed":["20123736"],"doi":["10.1093/nar/gkq017"]}}