biostudies-literatureUnknown76(12)Kovacs ATBacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.Applied and environmental microbiology4085-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC2893497biostudies-literatureGenetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.PMC2893497van Hartskamp Mvan Kranenburg RKovacs ATKuipers OPfalseGenetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.2010-01-01T00:00:00Z2010 Jun2021-02-19T10:33:55Z2019-03-27T00:31:57ZS-EPMC28934972040055510.1128/AEM.03060-0910.1128/aem.03060-09