biostudies-literatureTan TGIntramural NIH HHSNIAID NIH HHS3977-89https://www.ebi.ac.uk/biostudies/studies/S-EPMC2895808biostudies-literatureUnknown28(23)Toxoplasma gondii is an intracellular parasite that causes severe neurologic and ocular disease in immune-compromised and congenitally infected individuals. There is no vaccine protective against human toxoplasmosis. Herein, immunization of L(d) mice with HF10 (HPGSVNEFDF) with palmitic acid moieties or a monophosphoryl lipid A derivative elicited potent IFN-gamma production from L(d)-restricted CD8(+) T cells in vitro and protected mice. CD8(+) T cell peptide epitopes from T. gondii dense granule proteins GRA 3, 6, 7, and Sag 1, immunogenic in humans for HLA-A02(+), HLA-A03(+), and HLA-B07(+) cells were identified. Since peptide repertoire presented by MHC class I molecules to CD8(+) T cells is shaped by endoplasmic reticulum-associated aminopeptidase (ERAAP), polymorphisms in the human EVaccineIdentification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans.PMC2895808R01 AI027530R01 AI071319U01 AI77887U01 AI077887McLeod RTan TGSette AMui EWitola WHCong HMaewal AMontpetit AMuench SPGrigg MESidney JAlexander JfalseIdentification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans.Toxoplasma gondii is an intracellular parasite that causes severe neurologic and ocular disease in immune-compromised and congenitally infected individuals. There is no vaccine protective against human toxoplasmosis. Herein, immunization of L(d) mice with HF10 (HPGSVNEFDF) with palmitic acid moieties or a monophosphoryl lipid A derivative elicited potent IFN-gamma production from L(d)-restricted CD8(+) T cells in vitro and protected mice. CD8(+) T cell peptide epitopes from T. gondii dense granule proteins GRA 3, 6, 7, and Sag 1, immunogenic in humans for HLA-A02(+), HLA-A03(+), and HLA-B07(+) cells were identified. Since peptide repertoire presented by MHC class I molecules to CD8(+) T cells is shaped by endoplasmic reticulum-associated aminopeptidase (ERAAP), polymorphisms in the human E2010-01-01T00:00:00Z2010 May2026-04-16T21:21:49.22Z2026-04-07T14:17:43.879ZS-EPMC28958082034763010.1016/j.vaccine.2010.03.028