{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["76(13)"],"submitter":["Salazar E"],"pubmed_abstract":["The NifA-RpoN complex is a master regulator of the nitrogen fixation genes in alphaproteobacteria. Based on the complete Rhizobium etli genome sequence, we constructed an R. etli CFN42 oligonucleotide (70-mer) microarray and utilized this tool, reverse transcription (RT)-PCR analysis (transcriptomics), proteomics, and bioinformatics to decipher the NifA-RpoN regulon under microaerobic conditions (free life) and in symbiosis with bean plants. The R. etli NifA-RpoN regulon was determined to contain 78 genes, including the genes involved in nitrogen fixation, and the analyses revealed 42 new NifA-RpoN-dependent genes. More importantly, this study demonstrated that the NifA-RpoN regulon is composed of genes and proteins that have very diverse functions, that play fundamental and previously les"],"journal":["Applied and environmental microbiology"],"pagination":["4510-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2897426"],"repository":["biostudies-literature"],"pubmed_title":["Characterization of the NifA-RpoN regulon in Rhizobium etli in free life and in symbiosis with Phaseolus vulgaris."],"pmcid":["PMC2897426"],"pubmed_authors":["Salazar E","Moreno-Hagelsieb G","Martinez-Batallar G","Diaz-Mejia JJ","Encarnacion S","Mora Y","Mora J"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of the NifA-RpoN regulon in Rhizobium etli in free life and in symbiosis with Phaseolus vulgaris.","description":"The NifA-RpoN complex is a master regulator of the nitrogen fixation genes in alphaproteobacteria. Based on the complete Rhizobium etli genome sequence, we constructed an R. etli CFN42 oligonucleotide (70-mer) microarray and utilized this tool, reverse transcription (RT)-PCR analysis (transcriptomics), proteomics, and bioinformatics to decipher the NifA-RpoN regulon under microaerobic conditions (free life) and in symbiosis with bean plants. The R. etli NifA-RpoN regulon was determined to contain 78 genes, including the genes involved in nitrogen fixation, and the analyses revealed 42 new NifA-RpoN-dependent genes. More importantly, this study demonstrated that the NifA-RpoN regulon is composed of genes and proteins that have very diverse functions, that play fundamental and previously les","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jul","modification":"2026-05-02T14:45:11.803Z","creation":"2019-03-27T00:32:08Z"},"accession":"S-EPMC2897426","cross_references":{"pubmed":["20453139"],"doi":["10.1128/aem.02007-09","10.1128/AEM.02007-09"]}}