{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sindhava V"],"funding":["NIAID NIH HHS","NCI NIH HHS"],"pagination":["e11445"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2897882"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(7)"],"pubmed_abstract":["B cells are typically characterized as positive regulators of the immune response, primarily by producing antibodies. However, recent studies indicate that various subsets of B cells can perform regulatory functions mainly through IL-10 secretion. Here we discovered that peritoneal B-1 (B-1P) cells produce high levels of IL-10 upon stimulation with several Toll-like receptor (TLR) ligands. High levels of IL-10 suppressed B-1P cell proliferation and differentiation response to all TLR ligands studied in an autocrine manner in vitro and in vivo. IL-10 that accumulated in cultures inhibited B-1P cells at second and subsequent cell divisions mainly at the G1/S interphase. IL-10 inhibits TLR induced B-1P cell activation by blocking the classical NF-kappaB pathway. Co-stimulation with CD40 or BAFF abrogated the IL-10 inhibitory effect on B-1P cells during TLR stimulation. Finally, B-1P cells adoptively transferred from the peritoneal cavity of IL-10(-/-) mice showed better clearance of Borrelia hermsii than wild-type B-1P cells. This study described a novel autoregulatory property of B-1P cells mediated by B-1P cell derived IL-10, which may affect the function of B-1P cells in infection and autoimmunity."],"journal":["PloS one"],"pubmed_title":["Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection."],"pmcid":["PMC2897882"],"funding_grant_id":["CA09357","R21 AI076956","T32 CA009357","AI076956"],"pubmed_authors":["Bondada S","Sindhava V","Stevenson B","Woodman ME"],"additional_accession":[]},"is_claimable":false,"name":"Interleukin-10 mediated autoregulation of murine B-1 B-cells and its role in Borrelia hermsii infection.","description":"B cells are typically characterized as positive regulators of the immune response, primarily by producing antibodies. However, recent studies indicate that various subsets of B cells can perform regulatory functions mainly through IL-10 secretion. Here we discovered that peritoneal B-1 (B-1P) cells produce high levels of IL-10 upon stimulation with several Toll-like receptor (TLR) ligands. High levels of IL-10 suppressed B-1P cell proliferation and differentiation response to all TLR ligands studied in an autocrine manner in vitro and in vivo. IL-10 that accumulated in cultures inhibited B-1P cells at second and subsequent cell divisions mainly at the G1/S interphase. IL-10 inhibits TLR induced B-1P cell activation by blocking the classical NF-kappaB pathway. Co-stimulation with CD40 or BAFF abrogated the IL-10 inhibitory effect on B-1P cells during TLR stimulation. Finally, B-1P cells adoptively transferred from the peritoneal cavity of IL-10(-/-) mice showed better clearance of Borrelia hermsii than wild-type B-1P cells. This study described a novel autoregulatory property of B-1P cells mediated by B-1P cell derived IL-10, which may affect the function of B-1P cells in infection and autoimmunity.","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jul","modification":"2020-11-20T09:47:42Z","creation":"2019-03-26T23:08:48Z"},"accession":"S-EPMC2897882","cross_references":{"pubmed":["20625435"],"doi":["10.1371/journal.pone.0011445"]}}