biostudies-literatureUnknown11Revil TCanadian Institutes of Health Research<h4>Background</h4>Alternative splicing is known to increase the complexity of mammalian transcriptomes since nearly all mammalian genes express multiple pre-mRNA isoforms. However, our knowledge of the extent and function of alternative splicing in early embryonic development is based mainly on a few isolated examples. High throughput technologies now allow us to study genome-wide alternative splicing during mouse development.<h4>Results</h4>A genome-wide analysis of alternative isoform expression in embryonic day 8.5, 9.5 and 11.5 mouse embryos and placenta was carried out using a splicing-sensitive exon microarray. We show that alternative splicing and isoform expression is frequent across developmental stages and tissues, and is comparable in frequency to the variation in whole-transcrBMC genomics399https://www.ebi.ac.uk/biostudies/studies/S-EPMC2898759biostudies-literatureAlternative splicing is frequent during early embryonic development in mouse.PMC2898759Revil TDias CGaffney DJerome-Majewska LAMajewski JfalseAlternative splicing is frequent during early embryonic development in mouse.<h4>Background</h4>Alternative splicing is known to increase the complexity of mammalian transcriptomes since nearly all mammalian genes express multiple pre-mRNA isoforms. However, our knowledge of the extent and function of alternative splicing in early embryonic development is based mainly on a few isolated examples. High throughput technologies now allow us to study genome-wide alternative splicing during mouse development.<h4>Results</h4>A genome-wide analysis of alternative isoform expression in embryonic day 8.5, 9.5 and 11.5 mouse embryos and placenta was carried out using a splicing-sensitive exon microarray. We show that alternative splicing and isoform expression is frequent across developmental stages and tissues, and is comparable in frequency to the variation in whole-transcr2010-01-01T00:00:00Z2010 Jun2026-04-15T03:14:18.412Z2026-04-15T03:09:32.31ZS-EPMC28987592057321310.1186/1471-2164-11-399