{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dey R"],"funding":["Intramural NIH HHS"],"pagination":["399-414"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2909329"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["77(2)"],"pubmed_abstract":["Leishmaniasis is caused by the dimorphic protozoan parasite Leishmania. Differentiation of the insect form, promastigotes, to the vertebrate form, amastigotes, and survival inside the vertebrate host accompanies a drastic metabolic shift. We describe a gene first identified in amastigotes that is essential for survival inside the host. Gene expression analysis identified a 27 kDa protein-encoding gene (Ldp27) that was more abundantly expressed in amastigotes and metacyclic promastigotes than in procyclic promastigotes. Immunofluorescence and biochemical analysis revealed that Ldp27 is a mitochondrial membrane protein. Co-immunoprecipitation using antibodies to the cytochrome c oxidase (COX) complex, present in the inner mitochondrial membrane, placed the p27 protein in the COX complex. Ldp"],"journal":["Molecular microbiology"],"pubmed_title":["Characterization of a Leishmania stage-specific mitochondrial membrane protein that enhances the activity of cytochrome c oxidase and its role in virulence."],"pmcid":["PMC2909329"],"funding_grant_id":["Z99 OD999999"],"pubmed_authors":["Meneses C","Nakhasi HL","Salotra P","Dey R","Kamhawi S","Duncan R"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of a Leishmania stage-specific mitochondrial membrane protein that enhances the activity of cytochrome c oxidase and its role in virulence.","description":"Leishmaniasis is caused by the dimorphic protozoan parasite Leishmania. Differentiation of the insect form, promastigotes, to the vertebrate form, amastigotes, and survival inside the vertebrate host accompanies a drastic metabolic shift. We describe a gene first identified in amastigotes that is essential for survival inside the host. Gene expression analysis identified a 27 kDa protein-encoding gene (Ldp27) that was more abundantly expressed in amastigotes and metacyclic promastigotes than in procyclic promastigotes. Immunofluorescence and biochemical analysis revealed that Ldp27 is a mitochondrial membrane protein. Co-immunoprecipitation using antibodies to the cytochrome c oxidase (COX) complex, present in the inner mitochondrial membrane, placed the p27 protein in the COX complex. Ldp","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jul","modification":"2025-04-26T21:01:07.978Z","creation":"2019-03-27T00:32:39Z"},"accession":"S-EPMC2909329","cross_references":{"pubmed":["20497506"],"doi":["10.1111/j.1365-2958.2010.07214.x"]}}