{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(7)"],"submitter":["Luang S"],"pubmed_abstract":["Wild-type and variant crystals of a recombinant enzyme beta-d-glucan glucohydrolase from barley (Hordeum vulgare L.) were obtained by macroseeding and cross-seeding with microcrystals obtained from native plant protein. Crystals grew to dimensions of up to 500 x 250 x 375 mum at 277 K in the hanging-drops by vapour-diffusion. Further, the conditions are described that yielded the wild-type crystals with dimensions of 80 x 40 x 60 mum by self-nucleation vapour-diffusion in sitting-drops at 281 K. The wild-type and recombinant crystals prepared by seeding techniques achived full size within 5-14 days, while the wild-type crystals grown by self-nucleation appeared after 30 days and reached their maximum size after another two months. Both the wild-type and recombinant variant crystals, the la"],"journal":["International journal of molecular sciences"],"pagination":["2759-69"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2920565"],"repository":["biostudies-literature"],"pubmed_title":["Crystallisation of wild-type and variant forms of a recombinant plant enzyme β-D-glucan glucohydrolase from barley (Hordeum vulgare L.) and preliminary X-ray analysis."],"pmcid":["PMC2920565"],"pubmed_authors":["Streltsov VA","Ketudat Cairns JR","Hrmova M","Luang S"],"additional_accession":[]},"is_claimable":false,"name":"Crystallisation of wild-type and variant forms of a recombinant plant enzyme β-D-glucan glucohydrolase from barley (Hordeum vulgare L.) and preliminary X-ray analysis.","description":"Wild-type and variant crystals of a recombinant enzyme beta-d-glucan glucohydrolase from barley (Hordeum vulgare L.) were obtained by macroseeding and cross-seeding with microcrystals obtained from native plant protein. Crystals grew to dimensions of up to 500 x 250 x 375 mum at 277 K in the hanging-drops by vapour-diffusion. Further, the conditions are described that yielded the wild-type crystals with dimensions of 80 x 40 x 60 mum by self-nucleation vapour-diffusion in sitting-drops at 281 K. The wild-type and recombinant crystals prepared by seeding techniques achived full size within 5-14 days, while the wild-type crystals grown by self-nucleation appeared after 30 days and reached their maximum size after another two months. Both the wild-type and recombinant variant crystals, the la","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jul","modification":"2025-04-18T17:02:20.109Z","creation":"2019-03-27T00:33:13Z"},"accession":"S-EPMC2920565","cross_references":{"pubmed":["20717535"],"doi":["10.3390/ijms11072759"]}}