biostudies-literatureKarner CMNIDDK NIH HHSNIGMS NIH HHS2014-23https://www.ebi.ac.uk/biostudies/studies/S-EPMC2925040biostudies-literatureUnknown239(7)Recent studies using small molecule antagonists have revealed that the poly(ADP-ribose) polymerases (PARPs) Tankyrase 1 and 2 are critical regulators of canonical Wnt signaling in some cellular contexts. However, the absence of any activity during zebrafish embryogenesis suggested that the tankyrases may not be general/core components of the Wnt pathway. Here, we show that Tnks1 and 2 are broadly expressed during mouse development and are essential during kidney and lung development. In the kidney, blockage of tankyrase activity phenocopies the effect of blocking production of all Wnt ligands. Tankyrase inhibition can be rescued by activation of beta-catenin demonstrating its specificity for the Wnt pathway. In addition, treatment with tankyrase inhibitors appears to be completely reversibDevelopmental dynamics : an official publication of the American Association of AnatomistsTankyrase is necessary for canonical Wnt signaling during kidney development.PMC2925040R01DK080004R01GM079554R01 GM076398R01 GM079554R01GM076398R01 DK080004Merkel CELum LCarroll TJKarner CMDodge MLu JMa ZChen CfalseTankyrase is necessary for canonical Wnt signaling during kidney development.Recent studies using small molecule antagonists have revealed that the poly(ADP-ribose) polymerases (PARPs) Tankyrase 1 and 2 are critical regulators of canonical Wnt signaling in some cellular contexts. However, the absence of any activity during zebrafish embryogenesis suggested that the tankyrases may not be general/core components of the Wnt pathway. Here, we show that Tnks1 and 2 are broadly expressed during mouse development and are essential during kidney and lung development. In the kidney, blockage of tankyrase activity phenocopies the effect of blocking production of all Wnt ligands. Tankyrase inhibition can be rescued by activation of beta-catenin demonstrating its specificity for the Wnt pathway. In addition, treatment with tankyrase inhibitors appears to be completely reversib2010-01-01T00:00:00Z2010 Jul2026-04-07T17:39:04.091Z2019-03-27T00:33:27ZS-EPMC29250402054972010.1002/dvdy.22340