{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Muller CS"],"funding":["Austrian Science Fund FWF"],"pagination":["14950-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2930569"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["107(34)"],"pubmed_abstract":["Local Ca(2+) signaling occurring within nanometers of voltage-gated Ca(2+) (Cav) channels is crucial for CNS function, yet the molecular composition of Cav channel nano-environments is largely unresolved. Here, we used a proteomic strategy combining knockout-controlled multiepitope affinity purifications with high-resolution quantitative MS for comprehensive analysis of the molecular nano-environments of the Cav2 channel family in the whole rodent brain. The analysis shows that Cav2 channels, composed of pore-forming alpha1 and auxiliary beta subunits, are embedded into protein networks that may be assembled from a pool of approximately 200 proteins with distinct abundance, stability of assembly, and preference for the three Cav2 subtypes. The majority of these proteins have not previously"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Quantitative proteomics of the Cav2 channel nano-environments in the mammalian brain."],"pmcid":["PMC2930569"],"funding_grant_id":["P 20670"],"pubmed_authors":["Knaus HG","Schindler J","Muller CS","Striessnig J","Flockerzi V","Rammner B","Haupt A","Bildl W","Fakler B","Meissner M","Schulte U"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative proteomics of the Cav2 channel nano-environments in the mammalian brain.","description":"Local Ca(2+) signaling occurring within nanometers of voltage-gated Ca(2+) (Cav) channels is crucial for CNS function, yet the molecular composition of Cav channel nano-environments is largely unresolved. Here, we used a proteomic strategy combining knockout-controlled multiepitope affinity purifications with high-resolution quantitative MS for comprehensive analysis of the molecular nano-environments of the Cav2 channel family in the whole rodent brain. The analysis shows that Cav2 channels, composed of pore-forming alpha1 and auxiliary beta subunits, are embedded into protein networks that may be assembled from a pool of approximately 200 proteins with distinct abundance, stability of assembly, and preference for the three Cav2 subtypes. The majority of these proteins have not previously","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Aug","modification":"2025-07-24T03:04:43.53Z","creation":"2025-07-24T03:04:43.53Z"},"accession":"S-EPMC2930569","cross_references":{"pubmed":["20668236"],"doi":["10.1073/pnas.1005940107"]}}