{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Makitrynskyy R"],"funding":["NCRR NIH HHS","NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["559-66"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2939378"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(6)"],"pubmed_abstract":["Moenomycin, a natural phosphoglycolipid product that has a long history of use in animal nutrition, is currently considered an attractive starting point for the development of novel antibiotics. We recently reconstituted the biosynthesis of this natural product in a heterologous host, Streptomyces lividans TK24, but production levels were too low to be useful. We have examined several other streptomycetes strains as hosts and have also explored the overexpression of two pleiotropic regulatory genes, afsS and relA, on moenomycin production. A moenomycin-resistant derivative of S. albus J1074 was found to give the highest titers of moenomycin, and production was improved by overexpressing relA. Partial duplication of the moe cluster 1 in S. ghanaensis also increased average moenomycin produc"],"journal":["Journal of industrial microbiology & biotechnology"],"pubmed_title":["Genetic factors that influence moenomycin production in streptomycetes."],"pmcid":["PMC2939378"],"funding_grant_id":["R01 AI050855","P01 AI083214-026668","R01 GM076710-04","P41 RR015301-075426","R01 AI050855-06","P01 AI083214","R01 GM076710","AI50855"],"pubmed_authors":["Ostash B","Rabyk M","Makitrynskyy R","Rebets Y","Walker S","Zaburannyi N","Fedorenko V"],"additional_accession":[]},"is_claimable":false,"name":"Genetic factors that influence moenomycin production in streptomycetes.","description":"Moenomycin, a natural phosphoglycolipid product that has a long history of use in animal nutrition, is currently considered an attractive starting point for the development of novel antibiotics. We recently reconstituted the biosynthesis of this natural product in a heterologous host, Streptomyces lividans TK24, but production levels were too low to be useful. We have examined several other streptomycetes strains as hosts and have also explored the overexpression of two pleiotropic regulatory genes, afsS and relA, on moenomycin production. A moenomycin-resistant derivative of S. albus J1074 was found to give the highest titers of moenomycin, and production was improved by overexpressing relA. Partial duplication of the moe cluster 1 in S. ghanaensis also increased average moenomycin produc","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Jun","modification":"2025-04-05T00:26:43.418Z","creation":"2019-03-27T00:34:06Z"},"accession":"S-EPMC2939378","cross_references":{"pubmed":["20204454"],"doi":["10.1007/s10295-010-0701-1"]}}