biostudies-literatureContreras JNEI NIH HHSNIDDK NIH HHSNCI NIH HHS2735-47https://www.ebi.ac.uk/biostudies/studies/S-EPMC2948672biostudies-literatureUnknown4(5)In this study, nanoparticles based on difluoroboron dibenzoylmethane-poly(lactic acid) (BF(2)dbmPLA) are prepared. Polylactic acid or polylactide is a commonly used degradable polymer, while the boron dye possesses a large extinction coefficient, high emission quantum yield, two-photon absorption, and sensitivity to the surrounding environment. BF(2)dbmPLA exhibits molecular-weight-dependent emission properties and can be formulated as stable nanoparticles, suggesting that its unique optical properties may be useful in multiple contexts for probing intracellular environments. Here we show that BF(2)dbmPLA nanoparticles are internalized into cultured HeLa cells by endocytosis, and that within the cellular milieu, they retain their fluorescence properties. BF(2)dbmPLA nanoparticles are photoACS nanoIntracellular uptake and trafficking of difluoroboron dibenzoylmethane-polylactide nanoparticles in HeLa cells.PMC2948672R01 EY017293P30 CA4459F31 EY018807R01EY017293R01 EY017293-04S1P30 DK048522P30 DK048522-12F31EY018807P30 DK048522-14P30 DK048522-13R01 EY017293-03R01 EY017293-02S1R01 EY017293-04Zhang GXie JFraser CLChen YJPei HHamm-Alvarez SFContreras JfalseIntracellular uptake and trafficking of difluoroboron dibenzoylmethane-polylactide nanoparticles in HeLa cells.In this study, nanoparticles based on difluoroboron dibenzoylmethane-poly(lactic acid) (BF(2)dbmPLA) are prepared. Polylactic acid or polylactide is a commonly used degradable polymer, while the boron dye possesses a large extinction coefficient, high emission quantum yield, two-photon absorption, and sensitivity to the surrounding environment. BF(2)dbmPLA exhibits molecular-weight-dependent emission properties and can be formulated as stable nanoparticles, suggesting that its unique optical properties may be useful in multiple contexts for probing intracellular environments. Here we show that BF(2)dbmPLA nanoparticles are internalized into cultured HeLa cells by endocytosis, and that within the cellular milieu, they retain their fluorescence properties. BF(2)dbmPLA nanoparticles are photo2010-01-01T00:00:00Z2010 May2025-04-19T09:49:30.206Z2019-03-27T00:34:31ZS-EPMC29486722042041310.1021/nn901385y