biostudies-literatureDong XNIDDK NIH HHSPHS HHS1294-309https://www.ebi.ac.uk/biostudies/studies/S-EPMC2948974biostudies-literatureUnknown74(10)Acute urinary obstruction causes interstitial inflammation with leukocyte accumulation and the secretion of soluble mediators. Here we show that unilateral ureteral ligation caused a progressive increase in renal F4/80(+) and F4/80(-) dendritic cells, monocytes, neutrophils and T-cells 24-72 h following obstruction. Depletion of dendritic cells by clodronate pretreatment showed these cells to be the most potent source of tumor necrosis factor and other pro-inflammatory mediators in the obstructed kidney. F4/80(+) dendritic cells and T-cells co-localized in the cortico-medullary junction and cortex of the obstructed kidney. Cytokine secretion patterns and surface phenotypes of T-cells from obstructed kidneys were found to include interferon-gamma-secreting CD4(+) and CD8(+) memory T-cells aKidney internationalDendritic cells facilitate accumulation of IL-17 T cells in the kidney following acute renal obstruction.PMC2948974D47060R01 DK047060-10R01 DK068545-03DK68545R01 DK047060R01 DK068545Nath KAGriffin MDDong XMiller MNBachman LAfalseDendritic cells facilitate accumulation of IL-17 T cells in the kidney following acute renal obstruction.Acute urinary obstruction causes interstitial inflammation with leukocyte accumulation and the secretion of soluble mediators. Here we show that unilateral ureteral ligation caused a progressive increase in renal F4/80(+) and F4/80(-) dendritic cells, monocytes, neutrophils and T-cells 24-72 h following obstruction. Depletion of dendritic cells by clodronate pretreatment showed these cells to be the most potent source of tumor necrosis factor and other pro-inflammatory mediators in the obstructed kidney. F4/80(+) dendritic cells and T-cells co-localized in the cortico-medullary junction and cortex of the obstructed kidney. Cytokine secretion patterns and surface phenotypes of T-cells from obstructed kidneys were found to include interferon-gamma-secreting CD4(+) and CD8(+) memory T-cells a2008-01-01T00:00:00Z2008 Nov2025-04-19T09:50:02.091Z2019-03-26T23:04:46ZS-EPMC29489741897476010.1038/ki.2008.394