{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Warren M"],"funding":["NIBIB NIH HHS","NHLBI NIH HHS"],"pagination":["H1271-81"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2957348"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["299(4)"],"pubmed_abstract":["The use of voltage-sensitive fluorescent dyes (VSD) for noninvasive measurement of the action potential (AP) in isolated cells has been hindered by low-photon yield of the preparation, dye toxicity, and photodynamic damage. Here we used a new red-shifted VSD, di-4-ANBDQBS, and a fast electron-multiplied charge-coupled device camera for optical AP (OAP) recording in guinea pig cardiac myocytes. Loading di-4-ANBDQBS did not alter APs recorded with micropipette. With short laser exposures (just enough to record one OAP every 1-5 min), di-4-ANBDQBS yielded fluorescent signals with very high signal-to-background ratios (change in fluorescence on depolarization/fluorescence at resting potential: 19.2 ± 4.1%) and signal-to-noise ratios (40 ± 13.2). Quantum chemical calculations comparing the ANBD"],"journal":["American journal of physiology. Heart and circulatory physiology"],"pubmed_title":["High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS."],"pmcid":["PMC2957348"],"funding_grant_id":["2R01EB001963","5R37HL042873","R01 EB001963","5R01HL088444","R01 HL088444","R01 EB001963-27"],"pubmed_authors":["Warren M","Rees TD","Zaitsev AV","Steadman BW","Yan P","Loew LM","Venable P","Taylor T","Shibayama J","Spitzer KW","Wuskell JP"],"additional_accession":[]},"is_claimable":false,"name":"High-precision recording of the action potential in isolated cardiomyocytes using the near-infrared fluorescent dye di-4-ANBDQBS.","description":"The use of voltage-sensitive fluorescent dyes (VSD) for noninvasive measurement of the action potential (AP) in isolated cells has been hindered by low-photon yield of the preparation, dye toxicity, and photodynamic damage. Here we used a new red-shifted VSD, di-4-ANBDQBS, and a fast electron-multiplied charge-coupled device camera for optical AP (OAP) recording in guinea pig cardiac myocytes. Loading di-4-ANBDQBS did not alter APs recorded with micropipette. With short laser exposures (just enough to record one OAP every 1-5 min), di-4-ANBDQBS yielded fluorescent signals with very high signal-to-background ratios (change in fluorescence on depolarization/fluorescence at resting potential: 19.2 ± 4.1%) and signal-to-noise ratios (40 ± 13.2). Quantum chemical calculations comparing the ANBD","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Oct","modification":"2025-04-18T15:50:47.04Z","creation":"2019-03-27T00:34:58Z"},"accession":"S-EPMC2957348","cross_references":{"pubmed":["20601458"],"doi":["10.1152/ajpheart.00248.2010"]}}