biostudies-literatureUnknown63(4)Van Ooijen GRace-specific disease resistance in plants depends on the presence of resistance (R) genes. Most R genes encode NB-ARC-LRR proteins that carry a C-terminal leucine-rich repeat (LRR). Of the few proteins found to interact with the LRR domain, most have proposed (co)chaperone activity. Here, we report the identification of RSI2 (Required for Stability of I-2) as a protein that interacts with the LRR domain of the tomato R protein I-2. RSI2 belongs to the family of small heat shock proteins (sHSPs or HSP20s). HSP20s are ATP-independent chaperones that form oligomeric complexes with client proteins to prevent unfolding and subsequent aggregation. Silencing of RSI2-related HSP20s in Nicotiana benthamiana compromised the hypersensitive response that is normally induced by auto-active variants ofThe Plant journal : for cell and molecular biology563-72https://www.ebi.ac.uk/biostudies/studies/S-EPMC2988412biostudies-literatureThe small heat shock protein 20 RSI2 interacts with and is required for stability and function of tomato resistance protein I-2.PMC2988412Vossen JHTakken FLVan Ooijen GLukasik EVan Den Burg HACornelissen BJfalseThe small heat shock protein 20 RSI2 interacts with and is required for stability and function of tomato resistance protein I-2.Race-specific disease resistance in plants depends on the presence of resistance (R) genes. Most R genes encode NB-ARC-LRR proteins that carry a C-terminal leucine-rich repeat (LRR). Of the few proteins found to interact with the LRR domain, most have proposed (co)chaperone activity. Here, we report the identification of RSI2 (Required for Stability of I-2) as a protein that interacts with the LRR domain of the tomato R protein I-2. RSI2 belongs to the family of small heat shock proteins (sHSPs or HSP20s). HSP20s are ATP-independent chaperones that form oligomeric complexes with client proteins to prevent unfolding and subsequent aggregation. Silencing of RSI2-related HSP20s in Nicotiana benthamiana compromised the hypersensitive response that is normally induced by auto-active variants of2010-01-01T00:00:00Z2010 Aug2026-05-01T06:33:37.087Z2019-03-27T00:36:48ZS-EPMC29884122049738210.1111/j.1365-313X.2010.04260.x