{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["398(7-8)"],"submitter":["Heus F"],"pubmed_abstract":["One way to profile complex mixtures for receptor affinity is to couple liquid chromatography (LC) on-line to biochemical detection (BCD). A drawback of this hyphenated screening approach is the relatively high consumption of sample, receptor protein and (fluorescently labeled) tracer ligand. Here, we worked toward minimization of sample and reagent consumption, by coupling nano-LC on-line to a light-emitting diode (LED) based capillary confocal fluorescence detection system capable of on-line BCD with low-flow rates. In this fluorescence detection system, a capillary with an extended light path (bubble cell) was used as a detection cell in order to enhance sensitivity. The technology was applied to a fluorescent enhancement bioassay for the acetylcholine binding protein, a structural analo"],"journal":["Analytical and bioanalytical chemistry"],"pagination":["3023-32"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2990015"],"repository":["biostudies-literature"],"pubmed_title":["Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays."],"pmcid":["PMC2990015"],"pubmed_authors":["Kool J","Nahar TT","Heus F","Buijs J","Giera M","de Kloe GE","Niessen WM","Lingeman H","van Iperen D","Irth H","de Esch IJ","Smit AB"],"additional_accession":[]},"is_claimable":false,"name":"Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays.","description":"One way to profile complex mixtures for receptor affinity is to couple liquid chromatography (LC) on-line to biochemical detection (BCD). A drawback of this hyphenated screening approach is the relatively high consumption of sample, receptor protein and (fluorescently labeled) tracer ligand. Here, we worked toward minimization of sample and reagent consumption, by coupling nano-LC on-line to a light-emitting diode (LED) based capillary confocal fluorescence detection system capable of on-line BCD with low-flow rates. In this fluorescence detection system, a capillary with an extended light path (bubble cell) was used as a detection cell in order to enhance sensitivity. The technology was applied to a fluorescent enhancement bioassay for the acetylcholine binding protein, a structural analo","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Dec","modification":"2025-04-04T19:43:08.856Z","creation":"2019-03-27T00:36:50Z"},"accession":"S-EPMC2990015","cross_references":{"pubmed":["20872136"],"doi":["10.1007/s00216-010-4210-x"]}}