biostudies-literatureUnknown103(9)Goede V<h4>Background</h4>The combination of chemotherapy with the vascular endothelial growth factor (VEGF) antibody bevacizumab is a standard of care in advanced colorectal cancer (CRC). However, biomarkers predicting outcome of bevacizumab-containing treatment are lacking. As angiopoietin-2 (Ang-2) is a key regulator of vascular remodelling in concert with VEGF, we investigated its role as a biomarker in metastatic CRC.<h4>Methods</h4>Serum Ang-2 levels were measured in 33 healthy volunteers and 90 patients with CRC. Of these, 34 had metastatic disease and received bevacizumab-containing therapy. To determine the tissue of origin of Ang-2, quantitative real-time PCR was performed on microdissected cryosections of human CRC and in a murine xenograft model of CRC using species-specific amplificaBritish journal of cancer1407-14https://www.ebi.ac.uk/biostudies/studies/S-EPMC2990609biostudies-literatureIdentification of serum angiopoietin-2 as a biomarker for clinical outcome of colorectal cancer patients treated with bevacizumab-containing therapy.PMC2990609Koslowsky TCSchnell RCoutelle OReinacher-Schick AHacker UTOdenthal MSchmiegel WNeuneier JKashkar HHallek MWeihrauch MRGoede VCremer BAugustin HGfalseIdentification of serum angiopoietin-2 as a biomarker for clinical outcome of colorectal cancer patients treated with bevacizumab-containing therapy.<h4>Background</h4>The combination of chemotherapy with the vascular endothelial growth factor (VEGF) antibody bevacizumab is a standard of care in advanced colorectal cancer (CRC). However, biomarkers predicting outcome of bevacizumab-containing treatment are lacking. As angiopoietin-2 (Ang-2) is a key regulator of vascular remodelling in concert with VEGF, we investigated its role as a biomarker in metastatic CRC.<h4>Methods</h4>Serum Ang-2 levels were measured in 33 healthy volunteers and 90 patients with CRC. Of these, 34 had metastatic disease and received bevacizumab-containing therapy. To determine the tissue of origin of Ang-2, quantitative real-time PCR was performed on microdissected cryosections of human CRC and in a murine xenograft model of CRC using species-specific amplifica2010-01-01T00:00:00Z2010 Oct2025-04-04T19:42:35.42Z2019-03-27T00:36:51ZS-EPMC29906092092437210.1038/sj.bjc.6605925