biostudies-literatureZhang HNIGMS NIH HHS38324-36https://www.ebi.ac.uk/biostudies/studies/S-EPMC2992266biostudies-literatureUnknown285(49)Two novel regulatory motifs, LDEVFL and C-terminal regulatory Glu (E)-rich motif (CREEM), are identified in the extreme C terminus of the ABC protein DrrA, which is involved in direct interaction with the N-terminal cytoplasmic tail of the membrane protein DrrB and in homodimerization of DrrA. Disulfide cross-linking analysis showed that the CREEM and the region immediately upstream of CREEM participate directly in forming an interaction interface with the N terminus of DrrB. A series of mutations created in the LDEVFL and CREEM motifs drastically affected overall function of the DrrAB transporter. Mutations in the LDEVFL motif also significantly impaired interaction between the C terminus of DrrA and the N terminus of DrrB as well as the ability of DrrA and DrrB to co-purify, therefore suThe Journal of biological chemistryThe extreme C terminus of the ABC protein DrrA contains unique motifs involved in function and assembly of the DrrAB complex.PMC2992266R01 GM51981-09R01 GM051981Pradhan PZhang HKaur PfalseThe extreme C terminus of the ABC protein DrrA contains unique motifs involved in function and assembly of the DrrAB complex.Two novel regulatory motifs, LDEVFL and C-terminal regulatory Glu (E)-rich motif (CREEM), are identified in the extreme C terminus of the ABC protein DrrA, which is involved in direct interaction with the N-terminal cytoplasmic tail of the membrane protein DrrB and in homodimerization of DrrA. Disulfide cross-linking analysis showed that the CREEM and the region immediately upstream of CREEM participate directly in forming an interaction interface with the N terminus of DrrB. A series of mutations created in the LDEVFL and CREEM motifs drastically affected overall function of the DrrAB transporter. Mutations in the LDEVFL motif also significantly impaired interaction between the C terminus of DrrA and the N terminus of DrrB as well as the ability of DrrA and DrrB to co-purify, therefore su2010-01-01T00:00:00Z2010 Dec2026-04-30T23:27:47.586Z2019-03-27T00:36:55ZS-EPMC29922662087652710.1074/jbc.m110.13154010.1074/jbc.M110.131540