biostudies-literatureChu HWNIAID NIH HHSNHLBI NIH HHS155https://www.ebi.ac.uk/biostudies/studies/S-EPMC2992501biostudies-literatureUnknown11<h4>Background</h4>Respiratory infections including Mycoplasma pneumoniae (Mp) contribute to various chronic lung diseases. We have shown that mouse short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein was able to inhibit Mp growth. Further, airway epithelial cells increased SPLUNC1 expression upon Mp infection. However, the mechanisms underlying SPLUNC1 regulation remain unknown. In the current study, we investigated if SPLUNC1 production following Mp infection is regulated through Toll-like receptor 2 (TLR2) signaling.<h4>Methods</h4>Airway epithelial cell cultures were utilized to reveal the contribution of TLR2 signaling including NF-κB to SPLUNC1 production upon bacterial infection and TLR2 agonist stimulation.<h4>Results</h4>Mp and TLR2 agonist Pam3CSK4 increased SPLUNCRespiratory researchSPLUNC1 regulation in airway epithelial cells: role of Toll-like receptor 2 signaling.PMC2992501P01 HL073907R01 AI070175R01 HL088264Gally FZhang GCase SWu QMartin RJMichels NMinor MSmith SReisdorph NJiang DSimon GChu HWJanssen-Heininger YMThaikoottathil JfalseSPLUNC1 regulation in airway epithelial cells: role of Toll-like receptor 2 signaling.<h4>Background</h4>Respiratory infections including Mycoplasma pneumoniae (Mp) contribute to various chronic lung diseases. We have shown that mouse short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein was able to inhibit Mp growth. Further, airway epithelial cells increased SPLUNC1 expression upon Mp infection. However, the mechanisms underlying SPLUNC1 regulation remain unknown. In the current study, we investigated if SPLUNC1 production following Mp infection is regulated through Toll-like receptor 2 (TLR2) signaling.<h4>Methods</h4>Airway epithelial cell cultures were utilized to reveal the contribution of TLR2 signaling including NF-κB to SPLUNC1 production upon bacterial infection and TLR2 agonist stimulation.<h4>Results</h4>Mp and TLR2 agonist Pam3CSK4 increased SPLUNC2010-01-01T00:00:00Z2010 Nov2025-04-04T13:48:25.073Z2019-03-27T00:36:56ZS-EPMC29925012105486210.1186/1465-9921-11-155