biostudies-literatureRahman MMNIDDK NIH HHS678-90https://www.ebi.ac.uk/biostudies/studies/S-EPMC2993798biostudies-literatureUnknown2(10)O-linked-β-N-acetylglucosamine (O-GlcNAc) modification is a regulatory, nuclear and cytoplasmic post-translational glycosylation of proteins associated with age-related diseases such as Alzheimer's, Parkinson's, and type II diabetes. Global elevation of O-GlcNAc levels on intracellular proteins can induce insulin resistance, the hallmark of type II diabetes, in mammalian systems. InC. elegans, attenuation of the insulin-like signal transduction pathway increases adult lifespan of the nematode. We demonstrate that the O-GlcNAc cycling enzymes OGT and OGA, which add and remove O-GlcNAc respectively, modulate lifespan in C. elegans. Median adult lifespan is increased in an oga-1 deletion strain while median adult life span is decreased upon ogt-1 deletion. The O-GlcNAc-mediated effect on nemaAgingIntracellular protein glycosylation modulates insulin mediated lifespan in C.elegans.PMC2993798R01DK075069R01 DK075069Rahman MMEl-Karim EGStuart RStuchlick OKipreos ETWells LfalseIntracellular protein glycosylation modulates insulin mediated lifespan in C.elegans.O-linked-β-N-acetylglucosamine (O-GlcNAc) modification is a regulatory, nuclear and cytoplasmic post-translational glycosylation of proteins associated with age-related diseases such as Alzheimer's, Parkinson's, and type II diabetes. Global elevation of O-GlcNAc levels on intracellular proteins can induce insulin resistance, the hallmark of type II diabetes, in mammalian systems. InC. elegans, attenuation of the insulin-like signal transduction pathway increases adult lifespan of the nematode. We demonstrate that the O-GlcNAc cycling enzymes OGT and OGA, which add and remove O-GlcNAc respectively, modulate lifespan in C. elegans. Median adult lifespan is increased in an oga-1 deletion strain while median adult life span is decreased upon ogt-1 deletion. The O-GlcNAc-mediated effect on nema2010-01-01T00:00:00Z2010 Oct2025-04-26T05:23:35.527Z2019-03-27T00:37:00ZS-EPMC29937982095281110.18632/aging.100208