biostudies-literatureUnknown6Rennie B<h4>Background</h4>Bovine tuberculosis, caused by Mycobacterium bovis, afflicts approximately 50 million cattle worldwide and is detected by the tuberculin skin test (TST). While it has long been recognized that purified protein derivative (PPD) tuberculin is composed of a mixture of M. bovis derived protein components, little is known about the quality, relative quantity and identity of the proteins that make up PPD tuberculin. We manufactured a sterile filtered PPD tuberculin (SF-PPD) from a nine-week-old M. bovis culture supernatant in order to characterise the culture filtrate proteins (CFP) which make up M. bovis PPD tuberculin and to compare the antibody response of M. bovis infected versus M. bovis sensitized cattle.<h4>Results</h4>SF-PPD resolved into approximately 200 discrete spoBMC veterinary research50https://www.ebi.ac.uk/biostudies/studies/S-EPMC2994848biostudies-literatureAntibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle.PMC2994848Filion LGSmart NRennie BfalseAntibody response to a sterile filtered PPD tuberculin in M. bovis infected and M. bovis sensitized cattle.<h4>Background</h4>Bovine tuberculosis, caused by Mycobacterium bovis, afflicts approximately 50 million cattle worldwide and is detected by the tuberculin skin test (TST). While it has long been recognized that purified protein derivative (PPD) tuberculin is composed of a mixture of M. bovis derived protein components, little is known about the quality, relative quantity and identity of the proteins that make up PPD tuberculin. We manufactured a sterile filtered PPD tuberculin (SF-PPD) from a nine-week-old M. bovis culture supernatant in order to characterise the culture filtrate proteins (CFP) which make up M. bovis PPD tuberculin and to compare the antibody response of M. bovis infected versus M. bovis sensitized cattle.<h4>Results</h4>SF-PPD resolved into approximately 200 discrete spo2010-01-01T00:00:00Z2010 Nov2025-04-26T05:20:46.452Z2019-03-27T00:37:02ZS-EPMC29948482106248310.1186/1746-6148-6-50