{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fogle JE"],"funding":["NIAID NIH HHS"],"pagination":["97"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2997086"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["<h4>Background</h4>Using the FIV model, we reported previously that CD4+CD25+ T regulatory (Treg) cells from FIV+ cats are constitutively activated and suppress CD4+CD25- and CD8+ T cell immune responses. In an effort to further explore Treg-mediated suppression, we asked whether Treg cells induce anergy through the alteration of production of cyclins, cyclin-dependent kinases and their inhibitors.<h4>Results</h4>Lymphocytes were obtained from control or FIV+ cats and sorted by FACS into CD4+CD25+ and CD8+ populations. Following co-culture with CD4+CD25+ cells, CD8+ targets were examined by Western blot for changes in cyclins D3, E and A, retinoblastoma (Rb) protein, as well as the cyclin dependent kinase inhibitor p21cip1. Following co-culture with CD4+CD25+cells, we observed up-regulatio"],"journal":["Retrovirology"],"pubmed_title":["CD4+CD25+ T regulatory cells from FIV+ cats induce a unique anergic profile in CD8+ lymphocyte targets."],"pmcid":["PMC2997086"],"funding_grant_id":["R01 AI080288","AI080288","1K08AI074445"],"pubmed_authors":["Tompkins MB","Fogle JE","Tompkins WA"],"additional_accession":[]},"is_claimable":false,"name":"CD4+CD25+ T regulatory cells from FIV+ cats induce a unique anergic profile in CD8+ lymphocyte targets.","description":"<h4>Background</h4>Using the FIV model, we reported previously that CD4+CD25+ T regulatory (Treg) cells from FIV+ cats are constitutively activated and suppress CD4+CD25- and CD8+ T cell immune responses. In an effort to further explore Treg-mediated suppression, we asked whether Treg cells induce anergy through the alteration of production of cyclins, cyclin-dependent kinases and their inhibitors.<h4>Results</h4>Lymphocytes were obtained from control or FIV+ cats and sorted by FACS into CD4+CD25+ and CD8+ populations. Following co-culture with CD4+CD25+ cells, CD8+ targets were examined by Western blot for changes in cyclins D3, E and A, retinoblastoma (Rb) protein, as well as the cyclin dependent kinase inhibitor p21cip1. Following co-culture with CD4+CD25+cells, we observed up-regulatio","dates":{"release":"2010-01-01T00:00:00Z","publication":"2010 Nov","modification":"2025-04-18T16:35:41.917Z","creation":"2019-03-27T00:37:11Z"},"accession":"S-EPMC2997086","cross_references":{"pubmed":["21092106"],"doi":["10.1186/1742-4690-7-97"]}}