biostudies-literatureUnknown5(12)Miao AJThe behavior and toxicity of silver engineered nanoparticles (Ag-ENs) to the mixotrophic freshwater alga Ochromonas danica were examined in the present study to determine whether any other mechanisms are involved in their algal toxicity besides Ag(+) liberation outside the cells. Despite their good dispersability, the Ag-ENs were found to continuously aggregate and dissolve rapidly. When the initial nanoparticle concentration was lower than 10 µM, the total dissolved Ag(+) concentration ([Ag(+)](T)) in the suspending media reached its maximum after 1 d and then decreased suggesting that Ag(+) release might be limited by the nanoparticle surface area under these conditions. Furthermore, Ag-EN dissolution extent remarkably increased in the presence of glutathione. In the Ag-EN toxicity experPloS onee15196https://www.ebi.ac.uk/biostudies/studies/S-EPMC3008680biostudies-literatureIntracellular uptake: a possible mechanism for silver engineered nanoparticle toxicity to a freshwater alga Ochromonas danica.PMC3008680Chin WCQuigg ASantschi PHLuo ZMiao AJChen CSfalseIntracellular uptake: a possible mechanism for silver engineered nanoparticle toxicity to a freshwater alga Ochromonas danica.The behavior and toxicity of silver engineered nanoparticles (Ag-ENs) to the mixotrophic freshwater alga Ochromonas danica were examined in the present study to determine whether any other mechanisms are involved in their algal toxicity besides Ag(+) liberation outside the cells. Despite their good dispersability, the Ag-ENs were found to continuously aggregate and dissolve rapidly. When the initial nanoparticle concentration was lower than 10 µM, the total dissolved Ag(+) concentration ([Ag(+)](T)) in the suspending media reached its maximum after 1 d and then decreased suggesting that Ag(+) release might be limited by the nanoparticle surface area under these conditions. Furthermore, Ag-EN dissolution extent remarkably increased in the presence of glutathione. In the Ag-EN toxicity exper2010-01-01T00:00:00Z2010 Dec2026-05-29T10:52:36.003Z2026-04-08T04:48:45.501ZS-EPMC30086802120355210.1371/journal.pone.0015196