biostudies-literatureZong HNIDDK NIH HHS30-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC3012186biostudies-literatureUnknown60(1)<h4>Objective</h4>Previous studies have demonstrated that the VAMP8 protein plays a complex role in the control of granule secretion, transport vesicle trafficking, phagocytosis, and endocytosis. The present study was aimed to investigate the role of VAMP8 in mediating GLUT4 trafficking and therefore insulin action in mice.<h4>Research design and methods</h4>Physiological parameters were measured using Oxymax indirect calorimetry system in 12-week-old VAMP8 null mice. Dynamic analysis of glucose homeostasis was assessed using euglycemic-hyperinsulinemic clamp coupled with tracer radioactively labeled 2-deoxyglucose. Insulin stimulated GLUT4 protein expressions on muscle cell surface were examined by immunofluorescence microscopy.<h4>Results</h4>VAMP8 null mice display reduced adiposity witDiabetesEnhanced energy expenditure, glucose utilization, and insulin sensitivity in VAMP8 null mice.PMC3012186R01 DK058132-11DK-033823R37 DK033823P60 DK020541R01 DK082694P30 DK020541DK-020541R01 DK033823DK-082694Zong HVaitheesvaran BHong WWang CCPessin JEKurland IJfalseEnhanced energy expenditure, glucose utilization, and insulin sensitivity in VAMP8 null mice.<h4>Objective</h4>Previous studies have demonstrated that the VAMP8 protein plays a complex role in the control of granule secretion, transport vesicle trafficking, phagocytosis, and endocytosis. The present study was aimed to investigate the role of VAMP8 in mediating GLUT4 trafficking and therefore insulin action in mice.<h4>Research design and methods</h4>Physiological parameters were measured using Oxymax indirect calorimetry system in 12-week-old VAMP8 null mice. Dynamic analysis of glucose homeostasis was assessed using euglycemic-hyperinsulinemic clamp coupled with tracer radioactively labeled 2-deoxyglucose. Insulin stimulated GLUT4 protein expressions on muscle cell surface were examined by immunofluorescence microscopy.<h4>Results</h4>VAMP8 null mice display reduced adiposity wit2011-01-01T00:00:00Z2011 Jan2025-04-20T03:57:20.471Z2019-03-27T00:37:43ZS-EPMC30121862087671710.2337/db10-0231