{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ristic D"],"funding":["NCI NIH HHS"],"pagination":["155-67"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3017611"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(1)"],"pubmed_abstract":["The defining event in homologous recombination is the exchange of base-paired partners between a single-stranded (ss) DNA and a homologous duplex driven by recombinase proteins, such as human RAD51. To understand the mechanism of this essential genome maintenance event, we analyzed the structure of RAD51-DNA complexes representing strand exchange intermediates at nanometer resolution by scanning force microscopy. Joint molecules were formed between substrates with a defined ssDNA segment and homologous region on a double-stranded (ds) partner. We discovered and quantified several notable architectural features of RAD51 joint molecules. Each end of the RAD51-bound joints had a distinct structure. Using linear substrates, a 10-nt region of mispaired bases blocked extension of joint molecules"],"journal":["Nucleic acids research"],"pubmed_title":["Visualizing RAD51-mediated joint molecules: implications for recombination mechanism and the effect of sequence heterology."],"pmcid":["PMC3017611"],"funding_grant_id":["5P01CA092584","P01 CA092584"],"pubmed_authors":["Kanaar R","Ristic D","Wyman C"],"additional_accession":[]},"is_claimable":false,"name":"Visualizing RAD51-mediated joint molecules: implications for recombination mechanism and the effect of sequence heterology.","description":"The defining event in homologous recombination is the exchange of base-paired partners between a single-stranded (ss) DNA and a homologous duplex driven by recombinase proteins, such as human RAD51. To understand the mechanism of this essential genome maintenance event, we analyzed the structure of RAD51-DNA complexes representing strand exchange intermediates at nanometer resolution by scanning force microscopy. Joint molecules were formed between substrates with a defined ssDNA segment and homologous region on a double-stranded (ds) partner. We discovered and quantified several notable architectural features of RAD51 joint molecules. Each end of the RAD51-bound joints had a distinct structure. Using linear substrates, a 10-nt region of mispaired bases blocked extension of joint molecules","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jan","modification":"2025-04-04T19:18:16.357Z","creation":"2019-03-27T00:37:57Z"},"accession":"S-EPMC3017611","cross_references":{"pubmed":["20817928"],"doi":["10.1093/nar/gkq766"]}}