{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gourguechon S"],"funding":["PHS HHS"],"pagination":["142-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC3019802"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized."],"journal":["Eukaryotic cell"],"pubmed_title":["Rapid tagging and integration of genes in Giardia intestinalis."],"pmcid":["PMC3019802"],"funding_grant_id":["A1054693"],"pubmed_authors":["Gourguechon S","Cande WZ"],"additional_accession":[]},"is_claimable":false,"name":"Rapid tagging and integration of genes in Giardia intestinalis.","description":"We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.","dates":{"release":"2011-01-01T00:00:00Z","publication":"2011 Jan","modification":"2025-04-22T20:58:42.384Z","creation":"2019-03-27T00:38:01Z"},"accession":"S-EPMC3019802","cross_references":{"pubmed":["21115739"],"doi":["10.1128/EC.00190-10","10.1128/ec.00190-10"]}}